Fluorescence fluctuations microscopy

Fluorescence fluctuations analysis is derived from fluorescence correlation spectroscopy (FCS) and refers to a family of methods based on signal variations as fluorescent molecules or particles diffuse across a confines observation volume.  It can be applied to images acquired by a scanning microscope and provide absolute measurements of concentration and mobility of fluorescent molecules. We have developed such image correlation methods for different applications, such as the study of adhesion proteins in cells, in collaboration with biologists from Institute for Advances Biosciences (IAB), and the quantification of the surface density of proteins in biomimetic layers in collaboration with CEA/IRIG. 

References:

• De Mets et al., Molecular Biology of the Cell 30, 181 (2019).

• De Mets et al., Journal of Microscopy 279, 123 (2020).

• Sefkow-Werner et al., Anal. Chem. 94, 6521 (2022).

• Sefkow-Werner et al., ACS App. Mat. & Interfaces 14, 34113 (2022).