M2Bio : Microbiology and Molecular biology Platform

M2Bio is an experimental platform that accompanies you in the realization of your research projects requiring skills in molecular biology and / or microbiology (especially for phage display).
This service is open to LIPhy teams but also to external laboratories (consult us).

You will have access to the classical techniques :

• PCR
• DNA cloning
• Transformation and bacterial culture
• Production and extraction of plasmids
• Electrophoresis (agarose, SDS-PAGE)
• Dot blot, western blot, ELISA...

but also to a tailor-made accompaniment, according to your objective, in particular if your project requires more specific techniques:   

• Custom plasmid construction
• Modification of the E. Coli genome
• Insertion of reporter genes
• Fluorescence and optical density monitoring of bacterial cultures
• Use of M13 bacteriophages
• Phage display (ScFv or vH antibody libraries, peptides)
• Optimization of phage display screening according to targets (DNA, proteins, chemical molecules, polymers...)
• Evaluation of the specificity of hits...

We are members of the Bacteriophage France Network : https://site.phages.fr