M2Bio is an experimental platform that accompanies you in the realization of your research projects requiring skills in molecular biology and / or microbiology (especially for phage display).
This service is open to LIPhy teams but also to external laboratories (consult us).
You will have access to the classical techniques :
- PCR
- DNA cloning
- Transformation and bacterial culture
- Production and extraction of plasmids
- Electrophoresis (agarose, SDS-PAGE)
- Dot blot, western blot, ELISA...
but also to a tailor-made accompaniment, according to your objective, in particular if your project requires more specific techniques:
- Custom plasmid construction
- Modification of the E. Coli genome
- Insertion of reporter genes
- Fluorescence and optical density monitoring of bacterial cultures
- Use of M13 bacteriophages
- Phage display (ScFv or vH antibody libraries, peptides)
- Optimization of phage display screening according to targets (DNA, proteins, chemical molecules, polymers...)
- Evaluation of the specificity of hits...