Thesis defence

Description théorique et numérique de l'interaction des moteurs processifs et d'un microtubule

on the 31 January 2023
William Lecomte
Microtubules are key structures in cells, essential for cell division, intracellular transport and motility. 
These fine tubular structures, only a few micrometres in length, are subject to a non-equilibrium phenomenon known as dynamic instability, which consists of alternating phases of slow growth and rapid collapse. 
Although the last decades were devoted to the study of this phenomenon at the ends of the lattice, little is known about the dynamic processes along the shaft.
Recent in vitro observations, however, indicate a significant impact of processif motors on the dynamic processes and stability of the microtubule. 
Using a kinetic Monte Carlo method, I have developed a new theoretical model to describe the temporal evolution of a microtubule in the presence of a flow of processif motors that locally affect the structure of the lattice.
Among the many configurations that can be treated with this model, I focused in particular on the effect of kinesin-1 and yeast dynein on the microtubule lattice.
The results suggest that a small perturbation of the motors greatly accelerates dynamic processes such as microtubule breakage and remodelling.


LIPhy, salle de conférence
Mis à jour le 31 January 2023